Description
Product Information: DAS-ELISA
Citrus tristeza virus (CTV)
CTV (2) occurs worldwide wherever citrus is growing and is the economically most important viral disease
of citrus. It has a narrow range of hosts mostly restricted to citrus species. The virus is transmitted by
aphids in a semi-persistent manner. Important aphids are “the brown citrus aphid” (Toxoptera citricida)
and “the melon aphid” (Aphis gossypii). There is a great diversity of symptoms; the severity depends
on the virus strain and on the host (often also on the scion-rootstock combination). Symptoms include
declining (e.g. on sour orange rootstocks), seedling yellows, stem pitting, loss of vigour, wilting, stunting,
yellow-brown staining at bud union and on inner surface of bark, etc. up to complete die-back (e.g. of
limes irrespective of rootstocks).
Specificity and sampling instruction
Polyclonal antibodies were raised in rabbits against a CTV recombinant coat protein (6), enabling in
DAS-ELISA (3) the detection of mild as well as decline, seedling yellow and stem pitting isolates from
different parts of the world (broad-spectrum reagents). The concentration of CTV in tissue varies; thus,
conscious sample collection is important. Bark, petioles, and midribs of tender flushes, as well as pedicels
and buttons of fruits are good tissue sources. Field samples should be collected preferably in spring or
autumn, whereas sampling during prolonged hot weather should be avoided. Samples from 3-4 different
locations on the tree should be pooled (1,4,5). Samples are homogenized 1:20 (w/v) in extraction buffer
LABMED NEPAL offers the following formats:
Individual ELISA reagents for 96, 480 or 960 assays: IgG and/or conjugate for the working volume of
200 μl/test/well.
Reagent sets for 480 or 960 assays: IgG and conjugate, positive and negative controls, and microtiter
plates (F-96) for a working volume of 200 μl/test/well.
Complete kits for 96, 480 or 960 assays: All reagents, controls, microtiter plates (F-96), buffers, and
substrate necessary for a working volume of 200 μl/test/well.
ELISA buffers, equipment for sample preparation and disposables are also available.
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